Who we are
Where we are
The laboratory is located at the Institute of Human Anatomy, Department of Medicine and Surgery, 14 Gramsci Street, cross street 3C, 1st floor (SIPE code 1004).
The spaces are dedicated to carrying out experimental research, training and support for teaching activities.
What we do
The laboratory carries out basic and applied research in the field of studying the morphology and function of the hematopoietic system, consistent with the strategic lines of the Department.
The laboratory also supports the educational activities of the Department, in particular:
- conducting experimental dissertations of the Degree Programmes in Medicine and Surgery, Pharmaceutical, Veterinary and Medical biotechnologies, Translational Biomedical sciences and Biotechnologies,
- postgraduate training in PhD and Master's degrees.
Main lines of research
Research activities are focused on the study of morphological, phenotypic and functional aspects of cells of the hematopoietic system and their interactions with cytokines and cell differentiation factors.
In particular, the laboratory is concerned with:
- study of hematopoietic stem cell differentiation under physiological and pathological conditions;
- analysis of morphological-functional alterations and receptor phenotype of hematopoietic cells;
- characterization of intracellular signaling mechanisms;
- study of mechanisms of platelet production and function;
- phenotypic-functional characterization of lymphocyte populations.
Prof. Cecilia Carubbi: https://personale.unipr.it/it/ugovdocenti/person/99764
Prof. Elena Masselli: https://personale.unipr.it/it/ugovdocenti/person/90148
Prof. Prisco Mirandola: https://personale.unipr.it/it/ugovdocenti/person/21041
Prof. Giulia Pozzi: https://personale.unipr.it/it/ugovdocenti/person/180770
Prof. Orsola di Martino: https://personale.unipr.en/en/ugovdocenti/person/257743
Techniques in use
The facility has the following main technical expertise:
- flow cytometry;
- in vitro cell culture;
- modulation of gene expression by siRNA, shRNA, and CRISPR-Cas9;
- extraction and analysis of nucleic acids (RNA and DNA);
- RT-PCR and real-time PCR;
- Western blot;
- isolation of specific cell populations;
- histological and immunocytochemical techniques;
- light and fluorescence microscopy.
Main equipment provided
The laboratory is equipped with the following main equipment:
- 13-color flow cytometer (CytoFLEX, Beckman Coulter);
- AutoMACS Pro cell separator (Miltenyi Biotec);
- QuantStudio™ 1 Real-Time PCR System (Thermo Fisher Scientific);
- NanoDrop™ (Thermo Fisher Scientific);
- ChemiDoc™ Imaging System (Bio-Rad);
- Eclipse 80i Fluorescence Microscope (Nikon);
- Victor™ V3 Multilabel Counter microplate reader (PerkinElmer);
- Ultracentrifuge J20-XP (Beckman Coulter);
- Cytospin™ (Thermo Fisher Scientific);
- Rotary microtome for light microscopy;
- Ultramicrotome.
Integration with departmental activities
The facility operates in integration with the research and teaching activities of the Department of Medicine and Surgery, contributing to the quality of scientific production and the training of students and doctoral students.
