Laboratory of Hematology

Affiliating Staff:

- Prof. Nicola Giuliani; nicola.giuliani@unipr.it; +39 0521 033299
- Prof. Paola Storti; paola.storti@unipr.it; +39 0521 033301
- Dr.ssa Rosanna Vescovini; rosanna.vescovini@unipr.it; +39 0521 033270
- Dr. Denise Toscani; denise.toscani@unipr.it; +39 0521 033303
- Dott. Vincenzo Raimondi; vincenzo.raimondi@unipr.it; +39 0521 033303
- Dr. Nicolas Thomas Iannozzi; nicolasthomas.iannozzi@unipr.it; +39 0521 033303
- Dr. Mattia Dessena; mattia.dessena@unipr.it; +39 0521 033303
- Dr. Oxana Lungu; oxana.lungu@unipr.it; +39 0521 033303
- Dr. Camilla Sitzia; camilla.sitzia@unipr.it; +39 0521 033303
 

Pavilion 26, mezzanine floor

Via A. Gramsci 14

43126, Parma

.

Main Lines of Research

Study of the Pathophysiology of Multiple Myeloma and Pre-Malignant Monoclonal Gammapathies.
- Study of Molecular Alterations in Myeloma Cells and Its Microenvironment.
- Study of Immunological Alterations in Monoclonal Gammapathies.
- Study of Bone Marrow Angiogenesis and Pathophysiological Mechanisms Underlying the Pro-Angiogenic "Switch" in Multiple Myeloma.
- Pathophysiology of bone disease in Multiple Myeloma.
- Study of alterations in the metabolism of Myeloma cells and its bone marrow microenvironment.
- Identification of new diagnostic and prognostic markers in monoclonal gammapathies
- Identification of new therapeutic targets and study of new therapeutic strategies in Multiple Myeloma.
- Study of mesenchymal stem cells and the hematopoietic niche.
 

Main applied methodologies

- Gradient separation of mononuclear cells (Ficoll) and purification of cell subpopulations by "MACS" method from bone marrow and peripheral blood samples
- Classical and molecular cytogenetics
- Nucleic acid extraction
- Real-time PCR
- Microarrays
- Cytofluorimetry
- Apoptosis/proliferation assays
- ELISA
- Western Blot
- Immunofluorescence
- Single-cell RNA seq
 

Main equipment

- Biological hoods
- CO2 incubator
- Centrifuges
- FACSCelesta cytofluorometer
- NanodropOne
- Thermal cycler (T100 ThermalCycler)
- LightCycler480
- Electrophoresis and ChemiDoc Imaging System
- Optical and fluorescence microscopes with Genikon Acquisition and Analysis System
- Affymetrix Microarray Station
 

Keywords

- Multiple Myeloma
- Bone Marrow Microenvironment
- Cell Biology
- Molecular Biology
- Immunophenotype