Who we are
Scientific supervisor: Prof. Federica Rizzi
Technical supervisor: Dr.ssa Maria Giovanna Troglio
Contact information:
Relative staff
Where we are
Integrated Bio-tech complex Building B - Via Volturno 39 - 43125 Parma
What we do
Main lines of research
- Biological role of Clusterin (CLU) in normal and transformed cells.
- Identification of molecular biomarkers for prostate cancer diagnosis and prognosis prediction.
- Folate depletion and polyamine metabolism in prostate cancer.
- Molecular mechanisms of prostate cancer chemoprevention by green tea catechins.
- Tumor/ stroma cross-talk and exosome signaling effects on extracellular matrix remodeling and breast cancer progression.
- Study of the role of molecular chaperones in the formation kinetics and clearance process of protein aggregates, with special reference to the pathogenesis of Parkinson's Disease
- Evaluation of the effects from the application of innovative non-thermal technological treatments (e.g. gas plasma, plasma activated water) on different food matrices, by in vitro assays and in cellular models.
Techniques in use
- Cell cultures and definition of intracellular signaling pathways
- Extraction and quantification of nucleic acids, gene expression studies.
- Construction of expression vectors, cloning and production of recombinant proteins.
- Extraction, quantification of proteins and identification of proteins by hybridization techniques with antibodies (in chemiluminescence and fluorescence). Transmitted-light and fluorescence microscopy.
- Main cell Biology assays (viability, toxicity, proliferation, invasiveness, migration).
- Chromatin immunoprecipitation.
- Study of gene promoter activity.
- Enzyme assays in absorbance, fluorescence, chemiluminescence.
- Time-resolved fluorescence assays.
- ElISA assays.
- Cellular fractionation.
- HPLC
Keywords
Prostate cancer, polyamines, neurodegenerative diseases, chaperones, antioxidants and antioxidant capacity.
Main instrumentation provided
QuantStudioTM 3 Real-Time PCR System, Life Technologies.jpg
QuantStudioTM 3 Real-Time PCR System, Life Technologies:
The instrument enables amplification and quantification of genetic material with real-time fluorescent detection, ensuring high sensitivity and reproducibility of results. It is ideal for applications such as gene expression analysis, genotyping, genetic variation studies and detection of specific targets.
HPLC Shimaszu Prominance quaternario con rivelatore UV.jpg
HPLC Shimaszu Prominance quaternary HPLC with UV detector:
Low-Pressure Quaternary Gradient HPLC system equipped with UV detector
Allows the formation of Gradients of up to four solvents
(quaternary), settable from 0.0 to 100.0 % in 0.1 % increments
- Gradient accuracy < 0.50%
- Mixer volume variable between 10-500 μl
