Main lines of research:
Identification of molecular markers in biological fluids for the diagnosis and prognosis of cancer.
Identification of molecular markers in saliva and tissues for the early diagnosis of potentially malignant oral lesions.
Identification of differential molecular markers in in vitro models of breast cancer.
Hemocomponent research: analysis of blood component production processes and quality and study of the impact on planned therapeutic protocols with special focus on metabolic and inflammatory alterations.
Characterization and standardization of recombinant allergens for a contribution to molecular diagnostics of allergies and immunotherapy.
Structure-function relationships of biologically active peptides and interaction with biological membranes.
Protein-ligand interactions for drug development and optimization - SAR
Characterization and mechanism of action of retinol-transporting cellular proteins.
Study of the relationship between the concentration of biological markers and the health status of the digestive system
Techniques in use
High-resolution Nuclear Magnetic Resonance Spectroscopy, Circular Dicroism, Fluorescence, Dynamic Light Scattering.
Main instrumentation provided
Spectrofluorimeter, Spectrophotometer, mV Zetasizer (DLS), FPLC and recombinant protein production equipment.
Publications (2024-2025)
Gallo M, Ferrari E, Brugnoli F, Terrazzan A, Ancona P, Volinia S, Bertagnolo V, Bergamini CM, Spisni A, Pertinhez TA, Bianchi N. Metabolic Profiling of Breast Cancer Cell Lines: Unique and Shared Metabolites. Int J Mol Sci. 2025 Jan 24;26(3):969. doi: 10.3390/ijms26030969.
Artesani L, Gallo M, Giovati L, Bisignano FM, Ferrari E, Castronovo LM, Conti S, Santoro F, Pertinhez TA, Ciociola T. Anti-Staphylococcus aureus Activity and Structural Characterization of Rationally Designed Peptides. Antibiotics (Basel). 2025 Apr 26;14(5):437. doi: 10.3390/antibiotics14050437.
Ferrari E, Antonelli R, Gallo M, Meleti M, Setti G, Mucci A, Righi V, Gambini A, Magnoni C, Spisni A, Pertinhez TA. Salivary Metabolomics Discloses Metabolite Signatures of Oral Leukoplakia with and Without Dysplasia. Int J Mol Sci. 2025 Jul 7;26(13):6519. doi: 10.3390/ijms26136519.
Setti G, Gambini A, Righi V, Mucci A, Pertinhez TA, Ferrari E, Gallo M, Biscussi B, Antonelli R, Meleti M, Magnoni C. Tissue and Salivary NMR Metabolomics in Reticular-Type Oral Lichen Planus. NMR Biomed. 2025 Oct;38(10):e70137. doi: 10.1002/nbm.70137.
Agosta F, Pertinhez TA, Cozzini P, Spisni A, Ferrari E. Conformational Flexibility of a Lipocalin Allergen (Mus m 1): Implications for Molecular Allergy Diagnostics. Curr Issues Mol Biol. 2025 Mar 27;47(4):234. doi: 10.3390/cimb47040234.
Semelak JA, Gallo M, González Flecha FL, Di Pino S, Pertinhez TA, Zeida A, Gout I, Estrin DA, Trujillo M. Mg2+ binding to coenzyme A. Arch Biochem Biophys. 2025 Jan;763:110202. doi: 10.1016/j.abb.2024.110202.
Antonelli R, Ferrari E, Gallo M, Ciociola T, Calciolari E, Spisni A, Meleti M, Pertinhez TA. The Association between Salivary Metabolites and Gingival Bleeding Score in Healthy Subjects: A Pilot Study. Int J Mol Sci. 2024 May 17;25(10):5448. doi: 10.3390/ijms25105448.
