Who we are
Scientific supervisor:Prof. Enrico Fiaccadori
Technical supervisor:Dr. Silvana Pinelli
Info and contacts:Prof. Enrico Fiaccadori; enrico.fiaccadori@unipr.it; 0521-033182
Affiliating staff:
- Prof. Enrico Fiaccadori; enrico.fiaccadori@unipr.it; 0521-033182
- Prof. Umberto Maggiore; umberto.maggiore@unipr.it; 0521-702008
- Dr. Delsante Marco, Ph.D.; Hospital Medical Executive; marco.delsante@unipr.it
- Dr. ssa Gandolfini Ilaria, Ph.D.; Hospital Medical Executive; ilaria.gandolfini@unipr.it
- Dr. ssa Chiara Cantarelli, Hospital Medical Executive
- Dr. Giovanni Maria Rossi, Hospital Medical Executive
- Dr.ssa Isabella Pisani, Hospital Medical Executive
- Dr. Giuseppe Vizzini, Biomedical Laboratory Technician; gvizzini@ao.pr.it; 0521702018
- Dr. Chiara Italiano, Biomedical Laboratory Technician ; citaliano@ao.pr.it ; 0521702018
Where we are
Major Hospital of Parma, Via Gramsci 14
Hall 27, basement floor
What we do
- Kidney biopsy management and specimen preparation techniques for light microscopy and immunofluorescence. Particularly: acceptance, subdivision of the frustule using stereomicroscope, fixation of the frustules in the suitable fixatives and by liquid nitrogen, cutting of the sections by cryostat, preparation of immunofluorescence reaction by manual method.
- Storage of blood and urine samples for database management.
Main lines of research
- Clinicopathologic correlations in diabetic nephropathy
- New imaging methods for quantification of fibrosis in renal biopsy
- Use of artificial intelligence for morphoquantitative analysis of histologic lesions in renal biopsy
- Morphologic characterization of lesions in IgA nephropathy, global and segmental glomerulosclerosis, and monoclonal gammopathies of renal significance
- Histologic and ultrastructural lesion characterization in HIV-related nephropathy
- Oncogenic role of BK virus in renal transplant recipients
- Metabolism in genetically determined crystal nephropathies
- Donor antibodies-specific non-HLA antibodies in renal transplantation
- Recurrent post-transplantation nephropathies and glomerulopathies
Techniques in use
- Cutting frozen tissue sections by cryostat.
- Setting up direct and indirect immunofluorescence reaction by manual method.
- Second Harmonic Generation
- Inclusion of histological specimens in kerosene, cutting and staining with immunohistochemistry techniques and special histological staining
- Laser microdissection
Main instrumentation provided
- Stereomicroscope wild M3C (Heerbrugg Switzerland)
- Vibromix (PBI)
- ROTAMAG V12 (PROLABO)
- Leica Cryostat CM1860
- Leica Dialux 20EB Optical Microscope
- Leica DMLB Fluorescence Microscope
- Megafuge Centrifuge (Thermo Scinetific)
- Microdissector Laser Nikon Eclipse TE 2000U with mmi Cellcut
- Olympus BX41 optical microscope
- Cytomix FC500 cytofluorometer
- Luminex Biorad (BioPlex)
- Motic Scanner, histological slide scanner
Keywords: nephrology, nephropathology, renal biopsy, immunofluorescence, renal transplantation, glomerulonephritis
