Who we are
Scientific supervisor: Prof. Stefano Guizzardi
Technical supervisor: Dr.ssa Silvana Belletti
Info and contacts: Stefano Guizzardi; stefano.guizzardi@unipr.it; 0521 033917
Affiliating personnel:
Where we are
Integrated Bio-tech complex, Building A, Floor 1, 2
What we do
The Histology laboratory is concerned with the predominantly morphological and functional investigation of experimental approaches for tissue regeneration and healing using in vitro models. Lines of research include the evaluation of pharmacological compounds and biomedical devices such as biomaterials and the characterization of cellular responses to them.
Main lines of research:
- In vitro evaluation of the effect of Polynucleotides in cell growth and tissue repair processes.
- Evaluation of the anti-inflammatory effect of polynucleotides on periodontal cell population, comparative in vitro and in vivo study
- Investigation of chitosan scaffold functionalized as a substrate to promote epithelial regeneration, focusing especially on surface enrichment with active biomolecules to promote cell adhesion and scaffold colonization. Research also focuses on strategies for scaffold optimization, taking advantage of new available technologies such as 3D printing.
- Investigation of the cellular effects of pulsed electromagnetic fields (EMFs) in order to promote healing and regeneration of soft and mineralized tissues. Specifically, research focuses on synergistic interactions between EMF and biomolecules, optimizing treatment parameters in order to outline new protocols for bone regeneration.
Techniques in use
Cell cultures, primary cultures of bone cells and fibroblasts, cell viability assays, elisa assays, western blotting, cytotoxicity, classical histology, immunohistochemical and cytochemical investigations, immunofluorescence, digital image analysis, confocal microscopy on live cells and fixed preparations.
Main instrumentation provided:
Cell culture laboratory
- Nikon phase-contrast optical microscope with digital image acquisition system
- Laminar Flow Hood
- CO2 HERA incubator CELL (Hereus)
- Refrigerated Centrifuge Eppendorf 5804R
- Autoclave ANDROMEDA Plus (Tecnogaz)
- Bransonic 3510 tank sonicator
- Electronic analytical balance SCALTEC SBC33
- Microplate reader (fluorescence-absorbance-luminometry) Tecan Infinite F200
- Helios Gamma Spectrophotometer (Spectronic Unicam)
Histology Lab
- Histology Thermostat Stendifetta Bath Falc BI
- Inclusion Incubator Falc ITC
- Microtome Leitz 1512
- Microtome Reichert-Jung
- PH meter Crison 82200
- Wipa heating plate
- Electrical ventilated stove Memmert ULE 400
- Heron Chemistry hood
- Criostat 2700 Frigo- Cut Reichert-Jung
- Epifluorescence Microscope (Nikon Eclipse 80I) with digital image acquisition system
- Leitz Optical Microscope
- Stereo Microscope Herrbrugg Wild M3
- Ventilated Electric Stove Memmert BE 200
- Ultramicrotome LKB
- Glass Cutting Glass
- Transmission Electron Microscope Philips EMT 400
